Protein_Domain

Part:BBa_K112113:Design

Designed by: Bing Xia   Group: iGEM08_UC_Berkeley   (2008-10-21)


Ligase without start or stop codons


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1305
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1305
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1305
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 329


Design Notes

Source

PCR mv022/mv023 on pBca1256-K112109    (1489bp, EcoRI/BamHI/DpnI)
Sub into pBca1256                      (EcoRI/BamHI, 2472+910, L)
Product is pBca1256-K112113
-----------------------------------------------
mv022	Forward Biobricking of <ligase!
ccataGAATTCatgAGATCTattcttaaaattctgaacg
mv023	Reverse Biobricking of ligase>
cgttaGGATCCtagaccagttacctcatg

References